Inhibition of hapten‐specific cytotoxic T cell recognition by monoclonal anti‐hapten antibodies

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Abstract
The T cell‐mediated Cytotoxic response against autologous cells modified with the sulfhydryl reagent I‐AED (N‐iodoacetyl‐N′‐(5‐sulfonic‐l‐naphthyl) ethylene diamine) is hapten specific and H‐2 restricted (Levy, R. B., Shearer, G. M., Richardson, J. C. and Henkart, P. A., J. Immunol. 1981. 127: 523). We have produced a monoclonal antibody (V‐6‐3, IgM) which binds to AED‐modified cells and proteins. Competition experiments by free hapten indicated that the binding was AED specific. The effect of the mAb on AED‐specific cytotoxic T cell recognition at the effector and induction stage has been examined. Anti‐AED mAb inhibited the cell‐mediated lysis of some but not all AED‐specific, H‐2b‐restricted long‐term cytotoxic T cell clones and of bulk‐cultured C57BL/6 anti‐AED‐self effector cells. This blocking was not due to nonspecific agglutination of targets since lysis of AED‐modified target cells by alloreactive effector cells was not affected by this mAb under comparable conditions. Furthermore anti‐AED mAb specifically inhibited the antigen‐induced proliferation of AED‐specific long‐term cytotoxic T cell clones and the generation of AED‐specific cytotoxic effector cells in secondary cultures. This monoclonal anti‐AED antibody bound to cells modified by the recently described aminoreactive reagent AED‐NH2 (Takai, Y., Mizuochi, H., Fujiwava, H. and Hamaoka, T., J. Immunol. 1984. 132: 57); these same target cells were, however, not lysed by AED‐SH‐specific cytotoxic T cell clones.