Enzymatic and immunological characterization of the Mycobacterium fortuitum complex

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Abstract
The arylsulfatase isozymes of M. fortuitum, M. peregrinum, M. chelonei ssp. chelonei and M. chelonei ssp. abscessus were examined to determine the isozymal and immunological relationship among the members of the M. fortuitum complex. Cell extracts were subjected to electrophoresis on agarose and polyacrylamide gel, and arylsulfatase activity was localized using .beta.-naphthyl sulfate as substrate. Unique zymograms were produced for M. fortuitum, M. pererinum and M. chelonei which were characteristic for each species. The immunodiffusion and immunoelectrophoresis followed by sulfatase staining for the enzyme. One of the isozymes of M. fortuitum and M. peregrinum cross-reacted, showing immunological identity. Antisera to sulfatases of M. fortuitum and M. peregrinum did not react with sulfatases of M. chelonei. The characterization of sulfatase isozymes in extracts of organisms in the M. fortuitum complex suggests the division of the M. fortuitum complex into 2 spp., M. fortuitum and M. chelonei, with subspecies designations.