Plant disease resistance genes encode members of an ancient and diverse protein family within the nucleotide-binding superfamily
- 1 November 1999
- journal article
- Published by Wiley in The Plant Journal
- Vol. 20 (3) , 317-332
- https://doi.org/10.1046/j.1365-313x.1999.t01-1-00606.x
Abstract
The nucleotide binding site (NBS) is a characteristic domain of many plant resistance gene products. An increasing number of NBS-encoding sequences are being identified through gene cloning, PCR amplification with degenerate primers, and genome sequencing projects. The NBS domain was analyzed from 14 known plant resistance genes and more than 400 homologs, representing 26 genera of monocotyledonous, dicotyle-donous and one coniferous species. Two distinct groups of diverse sequences were identified, indicating divergence during evolution and an ancient origin for these sequences. One group was comprised of sequences encoding an N-terminal domain with Toll/Interleukin-1 receptor homology (TIR), including the known resistance genes, N, M, L6, RPP1 and RPP5. Surprisingly, this group was entirely absent from monocot species in searches of both random genomic sequences and large collections of ESTs. A second group contained monocot and dicot sequences, including the known resistance genes, RPS2, RPM1, I2, Mi, Dm3, Pi-B, Xa1, RPP8, RPS5 and Prf. Amino acid signatures in the conserved motifs comprising the NBS domain clearly distinguished these two groups. The Arabidopsis genome is estimated to contain approximately 200 genes that encode related NBS motifs; TIR sequences were more abundant and outnumber non-TIR sequences threefold. The Arabidopsis NBS sequences currently in the databases are located in approximately 21 genomic clusters and 14 isolated loci. NBS-encoding sequences may be more prevalent in rice. The wide distribution of these sequences in the plant kingdom and their prevalence in the Arabidopsis and rice genomes indicate that they are ancient, diverse and common in plants. Sequence inferences suggest that these genes encode a novel class of nucleotide-binding proteins.Keywords
This publication has 67 references indexed in Scilit:
- The domains of death: evolution of the apoptosis machineryTrends in Biochemical Sciences, 1999
- Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in ArabidopsisProceedings of the National Academy of Sciences, 1998
- Identification of R-Gene Homologous DNA Fragments Genetically Linked to Disease Resistance Loci in Arabidopsis thalianaMolecular Plant-Microbe Interactions®, 1998
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Signaling in Plant-Microbe InteractionsScience, 1997
- Inactivation of the flax rust resistance gene M associated with loss of a repeated unit within the leucine-rich repeat coding region.Plant Cell, 1997
- Plant Disease Resistance Genes: Function Meets Structure.Plant Cell, 1996
- Unsupervised Learning of Multiple Motifs in Biopolymers Using Expectation MaximizationMachine Learning, 1995
- RPS2 of Arabidopsis thaliana : a Leucine-Rich Repeat Class of Plant Disease Resistance GenesScience, 1994
- Nuclear DNA content of some important plant speciesPlant Molecular Biology Reporter, 1991