Determination of virginiamycin in chicken or swine tissues by high performance liquid chromatography.

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Abstract
A simple and rapid determination of virginiamycin (VGM) in chicken and swine tissues by quantitation of virginiamycin M1 (M1), which is a main component of VGM, was developed. M1 was extracted from the tissues with a mixture of methanol-3% phosphotungistic aci (7 : 3), and the extract was cleaned up by liquid-liquid partitioning with chloroform. The chloroform extract was further cleand up on a Sep-Pak silica cartridge using chloroform-methanol (97 : 3) as an eluent. M1 was determined by reversed phase HPLC which was performed on a Kaseisorb LC-ODS-300-5 column with acetonitrile-water (35 : 65) solution as a mobile phase, and M1 was monitored with UV detection at 235 nM. The caliblation curve was rectilinear in the range from 0.5 to 10.0 .mu.g/ml. The average recoveries were 93.6% and 95.2% for VGM added to chicken and swine tissues at the level of 1.0 .mu.g/g, respectivley. A good correlation obtained between the HPLC method and the bioassay method shows that the present method will be applicable to determine VGM with detection limit of 0.05 .mu.g/g.

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