PslD Is a Secreted Protein Required for Biofilm Formation by Pseudomonas aeruginosa
- 1 April 2006
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 72 (4) , 3066-8
- https://doi.org/10.1128/aem.72.4.3066-3068.2006
Abstract
The function of pslD , which is part of the psl operon from Pseudomonas aeruginosa , was investigated in this study. The psl operon is involved in exopolysaccharide biosynthesis and biofilm formation. An isogenic marker-free pslD deletion mutant of P. aeruginosa PAO1 which was deficient in the formation of differentiated biofilms was generated. Expression of only the pslD gene coding region restored the wild-type phenotype. A C-terminal, hexahistidine tag fusion enabled the identification of PslD. LacZ and PhoA translational fusions with PslD indicated that PslD is a secreted protein required for biofilm formation, presumably via its role in exopolysaccharide export.Keywords
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