THE INACTIVATION OF ANTITHROMBIN BY ETHER: EVIDENCE AGAINST THE EXISTENCE OF ANTITHROMBIN IV

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Abstract
Citrated defibrinated dog plasma was washed with ether to remove normal antithrombin. At least three washes with three volumes of ether for each wash were required to remove all antithrombin activity. Heparin-cofactor activity was also removed by this treatment. When the prothrombin of this ether-treated plasma was activated, the thrombin activity remained stable in the mixture for several hours. This is in contrast to activated normal plasma, in which the thrombin activity disappears within a few minutes because of the antithrombin content. By adding purified antithrombin to ether-treated plasma, a normal thrombin generation curve was obtained. It has been concluded that there is only one enzymatic antithrombin in plasma.

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