Resistance to Trypanosoma brucei in Mice Following Inoculation of NTG-Treated Trypanosomes

Abstract
The alkylating agent N-methyl-N′-nitro-N-nitrosoguanidine (NTG) was tested as an attenuating agent for Trypanosoma brucei. Parasites were treated with 4 mcg/ml NTG and injected into mice at intervals of 7–14 days. Those mice surviving four injections of NTG-treated trypanosomes were later challenged and showed a 100% resistance to infection. A corresponding group of control mice showed a 6% resistance to infection.

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