NUCLEOTIDE-SEQUENCE OF A FRAGMENT OF SV40 DNA THAT CONTAINS ORIGIN OF DNA-REPLICATION AND SPECIFIES 5' ENDS OF EARLY AND LATE VIRAL-RNA .3. CONSTRUCTION OF TOTAL SEQUENCE OF ECORII-G FRAGMENT OF SV40 DNA

  • 1 January 1977
    • journal article
    • research article
    • Vol. 252  (1) , 355-367
Abstract
Limited T1 RNase digestion of subfragments of the SV40 DNA restriction endonuclease fragment EcoRII-G were prepared and analyzed. The fragments were separately labeled with 32P at their 5'' terminus and the terminal sequences analyzed with limited snake venom diesterase digestion. The data permitted deduction of the nucleotide sequence for EcoRII-G. The sequence contains a stretch of 17 A-T base pairs preceding the DNA complementary to the 5'' end of early mRNA, a stretch of 27 bases with a perfect 2-fold rotational symmetry near the origin of DNA replication and a perfect tandem repeat of 21 nucleotides.

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