β-Glucanases in the yeast Cryptococcus albidus var. aerius. Production and separation of β-glucanases in asynchronous cultures

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Abstract
β-Glucanases were detected in cell-free extracts of the yeast Cryptococcus albidus var. aerius when grown on glucose as the sole carbon source. The production of β-glucanases was followed in log-phase cells and stationary-phase cells; the maximal production of β-(1 → 3) and β-(1 → 6) glucanases takes place respectively in log-phase and stationary-phase cells. The results show that there are marked differences in the elution profiles on Sephadex G-50 of fractions containing β-glucanase from cells grown for 12, 24, 48, 72, and 96 h. The possibility either of replacement changes in fractions containing β-glucanase activity or of a different synthesis of each β-glucanase during the growth of the yeast is discussed. The results suggest that all fractions containing β-glucanases hydrolyze both β-(1 → ) and β-(1 → 6) linkages.Evidence in support of the conclusion that a low molecular form of β-glucanase has a molecular weight of 2100 ± 100 is also shown.

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