Use of Synchronous Scan Excitation Spectra to Detect Fluorescent Metal Organic Ligands Produced by the Marine Diatom Phaeodactylum Tricornutum
- 1 January 1996
- journal article
- research article
- Published by Taylor & Francis in Environmental Technology
- Vol. 17 (1) , 25-33
- https://doi.org/10.1080/09593331708616357
Abstract
The use of synchronous scan excitation spectra (SSES) for investigating the fluorescence characteristics of the Sep-Pak extractable phytoplanktonic organic matter (SPE-POM) produced by the marine diatom Phaeodactylum tricornutum under (SPE-POM∗) and without (SPE-POM) metal stress is proposed. A Δλ of 70 nm was found suitable to detect the fluorophores occurring in such samples. A proteinaceous, lumichrome and lumiflavin contributions together with that of a degradation and/or condensation product were observed. The fluorescence quenching technique applied to SSES enabled detection of the contribution of each group of fluorophores to the copper complexation. The log K of complexes were in the range 5–6. A comparison with the Sep-Pak extractable marine organic matter (SPE-MOM) from seawater shows a relationship between the proteinaceous material produced by Phaeodactylum tricornutum and that occurring in seawater.Keywords
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