Candida albicans and three other Candida species contain an elongation factor structurally and functionally analogous to elongation factor 3

1 May 1991

journal article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 80 (1) , 45-49
https://doi.org/10.1016/0378-1097(91)90207-q

Abstract

A cell-free poly(U)-dependent translation elongation system from Candida albicans is ATP-dependent due to the presence of an elongation factor 3 (EF3)-like activity. Saccharomyces cerevisiae ribosomes added to a C. albicans post-ribosomal supernatant (PRS) supported poly(U)-dependent elongation, suggesting that the C. albicans lysate contained a soluble translation factor functionally analogous to the S. cerevisiae translation factor EF-3. The presence of EF-3 in C. albicans was confirmed by Western blotting using an antibody raised against S. cerevisiae EF-3. This antibody was also used to screen a selection of Candida species, all of which possessed EF-3 with molecular mass in the range of 110–130 kDa.

Keywords

This publication has 0 references indexed in Scilit: