Calmodulin Antagonist W‐7 Inhibits Lysosomal Sphingomyelinase Activity in C6 Glioma Cells
- 1 May 1989
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 52 (5) , 1645-1647
- https://doi.org/10.1111/j.1471-4159.1989.tb09221.x
Abstract
N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7) is known to be a potent calmodulin antagonist and inhibitor of calmodulin-dependent protein kinases. W-7 and 1-(5-isoquinolinyl-sulfonyl)-2-methylpiperazine (H-7) are inhibitors of protein kinase C and cyclic nucleotide-dependent protein kinases. In C6 glioma cells, W-7 and not H-7 inhibited dose-dependently acid sphingomyelinase, a result indicating the modulation of this lysosomal enzyme by a calmodulin-dependent system. Other lysosoml enzymes, such as .beta.-glucosidase, .alpha.-galactosidase, and arylsulfatase A, were unaffected by W-7 and H-7, a finding indicating a selective effect of W-7 on sphingomyelinase.Keywords
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