Tubulin as a molecular component of coated vesicles.

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Abstract
Two proteins of 53,000 and 56,000 MW were found to be associated with coated vesicles (CV) purified from bovine brain and chicken liver. These proteins share MW, isoelectric points and antigenic determinants with .alpha.- and .beta.-tubulins purified from bovine brain. Based on SDS, PAGE [sodium dodecyl sulfate polyacrylamide gel electrophoresis] and EM analysis of controlled pore glass bead exclusion column fractions, both the tubulins and the major CV polypeptide clathrin were found to chromatograph as components of a single kinetic particle. In addition, tubulin and CV antigens assayed by a sensitive enzyme-linked-immunoadsorbent method eluted from the columns with constant stoichiometry. Tubulin apparently is a molecular component of coated vesicles.