Estimation of testosterone binding capacity in the serum with hydrophobic resin.
- 1 January 1976
- journal article
- research article
- Published by Japan Endocrine Society in Endocrinologia Japonica
- Vol. 23 (2) , 173-178
- https://doi.org/10.1507/endocrj1954.23.173
Abstract
A method for the estimation of the serum testosterone binding capacity (TeBC) using hydrophobic resin, Amberlite XAD-2, was developed. The serum was incubated with a saturating amount of 1,2-3H-testosterone at 15.degree. C, unbound testosterone was adsorbed to the resin, and the 3H-radioactivity remaining in the supernatant fluid was counted. Under these conditions, the normal levels and their standard deviations were 15.08 .+-. 3.39 ng/ml (n = 7) for male and 35.06 .+-. 3.56 ng/ml (n = 6) for female, respectively. Precision of the method was 6.29%. TeBC in the 3rd mo. of pregnancy was approximately 1.5 times more than that of non-pregnant women, and approximately 3-5 times in the 10th mo.This publication has 4 references indexed in Scilit:
- Testosterone-Binding Levels in the Serum of Women During the Normal Menstrual Cycle, Pregnancy, and the Post-partum PeriodJournal of Clinical Endocrinology & Metabolism, 1967
- Steroid-Protein Interaction with Particular Reference to Testosterone Binding by Human SerumJournal of Biological Chemistry, 1967
- PROTEIN BINDING OF CORTICOIDS STUDIED BY GEL FILTRATION*Journal of Clinical Investigation, 1962
- TRANSCORTIN: A CORTICOSTEROID-BINDING PROTEIN OF PLASMA*†Journal of Clinical Investigation, 1959