Laboratory Studies with WARF Anti-resistant on House Flies1

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Abstract
A strain of house flies, Musca domestica L., resistant to DDT and to various other insecticides was exposed for a number of generations to a mixture of DDT and N , N dibutyl-p-chlorobenzenesulfonamide, a DDT synergist available under the name of WARF anti-resistant. Initially, the flies were considerably more susceptible to the mixture of DDT and synergist than to DDT alone. With repeated exposure to the combination, however, resistance developed which was detected after 3 generations of selection. In addition, there was cross resistance to the amyl analog of WARF anti-resistant and to the DDT synergist bis-(p-chlorophenyl) ethynyl carbinol. With the use of this mixture, diazinon resistance present initially in the strain was intensified greatly during the course of selection.

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