A comparative study on the measurement of urinary kallikrein in the rat.

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Abstract
The present study was carried out to assess the correlations among the results of four different assays of urinary kallikrein in the rat. The peptidase, esterase, vasodilator and kinin-forming activities were employed for the determination of kallikrein activity. In the urine from normal rats, the assay of vasodilator activity correlated well with those of peptidase activity (γ=0.86) and esterase activity (γ=0.89). Similarly, a good correlation (γ=0.99) was obtained between the peptidase and kinin-forming activities. However, in the case of rats under furosemide treatment, the esterase activity was poorly correlated with the peptidase and vasodilator activities, although a good correlation (γ=0.93) was obtained between the latter two activities. These results indicate that the peptidase and vasodilator activities should be employed for the estimation of urinary kallikrein excretion in the urine from rats under furosemide treatment.
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