A Rapid Technique for Inoculation ofPhaseolus vulgariswith Multiple Pathotypes ofUromyces phaseoli

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Abstract
Modification of a readily available, inexpensive, hand-held, pressurized spraying device was made by attaching a 5-cm long, 12-mm diameter, section of Plexiglas tubing over the spray nozzle. This modification permitted inoculation of bean plants with each of many pathotypes of U. phaseoli. Negligible differences in numbers of uredinia among pathotypes were obtained by standardizing the concentrations of viable urediniospores. Each unifoliolate leaf was inoculated with 4 pathotypes per leaf 6-8 days after seeding and the 1st trifoliolate leaf was inoculated with up to 6 pathotypes 6-8 days later. Resistant and susceptible host reactions to specific pathotypes were not affected by simultaneously or sequentially inoculating other leaf areas with other pathothypes. The rapid technique described is potentially useful for determining linkage relationships among host resistance genes and in breeding beans for resistance to multiple pathotypes of U. phaseoli.