Effect of cloned human interferon-? 2a on bovine parainfluenza-3 virus

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Abstract
Summary Pretreatment of bovine turbinate (BTu) cells with cloned human interferon (IFN)-α 2a reduced the yield of infectious bovine parainfluenza-3 virus (PI-3V). Intracellular synthesis of HN glycoprotein was reduced on postinfection day 1 (PID 1), but it recovered to normal levels subsequently. However, reduction of this protein persisted in the released virus through PID2. Thin section electron microscopy demonstrated a drastically reduced release of mature virions and an accumulation of viral nucleocapsids inside the cytoplasm on PID2. These results suggest that cloned human IFN-α 2a affects the glycoprotein synthesis, and morphogenesis of bovine PI-3V, and thus inhibits the release of viral particles from treated cells.