ISOTOPIC EXCHANGE IN FE-59 LABELED RICE-BASED MEALS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 90  (2) , 231-237
Abstract
The process of isotopic exchange between extrinsic 59Fe tracer and native nonheme Fe in a rice-based meal was studied by performing Sephadex G-50 column chromatography of soluble supernatant of 59Fe-labeled meal after in vitro pepsin digestion and duodenal aspirate obtained from a human subject fed a similarly labeled meal. The elution patterns of radioactivity and chemically determined nonheme Fe differed markedly when the supernatant of in vitro digested meal at pH 1.8 was analyzed; the patterns were similar when pH was adjusted to 6.2 and for duodenal aspirate at pH 7. Complete isotopic exchange between 59Fe tracer and native nonheme Fe in rice-based meal apparently does not take place in vitro or even during gastric digestion, but is probably completed in the duodenum.

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