High Pressure Liquid Chromatographic Determination of Mecillinam in Human Plasma and Urine

Publisher Website
Google Scholar
Abstract
A simple, specific, rapid and sensitive method for the analysis of mecillinam in plasma and urine using high pressure liquid chromatography (HPLC) is described. The assay is performed by direct injection of a plasma protein free supernatant or a dilution of urine. A .mu.Bondapak phenyl column with an eluting solvent of 16% CH3CN.sbd.0.2% H3PO4 was used, with UV detection of the effluent at 220 nm. Desacetylcephalothin was used as the internal standard and quantitation was based on peak height ratio of mecillinam to that of the internal standard. The lowest concentration detectable without extraction was 0.25 .mu.g/ml for plasma and 8.9 .mu.g/ml for urine. No interference from plasma and urine was noted. The assay was applied to the determination of plasma and urine concewntration of mecillinam following i.v. administration of a 10 mg/kg dose of mecillinam to 2 human subjects. The HPLC and microbiological assays correlated well for these samples.

This publication has 8 references indexed in Scilit: