Hemolysis of PR8-Virus Sensitized Erythrocytes.

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Abstract
A hemolytic system is descr. which involves the lysis of virus-antivirus sensitized chicken erythrocytes by complement and apparently depends upon specific interaction of virus and antivirus. Hemolytic curves resemble the usual erythrocyte-hemolysin hemolytic curves except that the slopes of the curves in the former increase upon dilution of antivirus. Activity of virus in such a system apparently follows that of the hemagglutinating particle. Inactivation of the virus at 56[degree]C for 30 min., which markedly inhibited elution at 37[degree]C, had no appreciable effect upon such hemolysis by complement in terms of the change in slopes. Changes in slopes were also not appreciably altered by previous adsorption of antivirus with a sedimentable component from pooled normal chorio-allantoic fluid.