Axenic Culture Studies of Entamoeba Species

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Abstract
Axenic Entamoeba invadens could be grown at room temperatures in the diphasic types of medium used for E. histolytica Conversely, axenic E. histolytica from diphasic cultures at ca. 37[degree]C, within limits, could be adapted into variations of the monophasic media originally developed for E. inyadens Only occasional E. histolytica inocula grew for more than 1 or 2 weekly subcultures in such monophasic media, but several series have been transferred up to 7 times. Of the various natural ingredients in E. histolytica media, only serum was required at consistently high levels (ca. 10%). E. invadens has been maintained for several weeks in chemically defined and partially defined media, but population increases were not observed. The survival of E. inva-dens but not E. histolytica was significantly extended by helping to preserve anaerobic conditions in cultures with a petroleum jelly seal. Zone electrophoresis in starch gel combined with chemically specific staining for enzymatic activity was used in an attempt to compare the esterase enzymes of the 2 axenically cultured species. However, only esterases associated with the culture media and not with the amebae themselves were demonstrated.

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