Sequence Determination of Peptide by the Combined Use of Fluoresceinisothiocyanate and Phenylisothiocyanate
- 1 August 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 42 (8) , 1559-1563
- https://doi.org/10.1080/00021369.1978.10863204
Abstract
A fluorometric manual technique has been demonstrated for determining the amino acid sequence of a polypeptide, by the combined use of fluoresceinisothiocyanate (FITC) and phenylisothiocyanate (PITC). This method permits continuous assignments of 10 residues with 5.6 nmoles of myoglobin or 23 residues with 45 nmoles.This publication has 11 references indexed in Scilit:
- A new method for the determination of N-terminus of peptides chain with fluorescein-isothiocyanatePublished by Elsevier ,2004
- Analysis of Fluorescein-thiohydantoin Amino Acids by High Speed Liquid ChromatographyAgricultural and Biological Chemistry, 1976
- Automatic procedures for determining amino acid sequences of peptidesAnalytical Biochemistry, 1975
- Automated microsequence analysis by use of radioactive phenylisothiocyanateAnalytical Biochemistry, 1975
- Rapid sequence analysis of small peptidesAnalytical Biochemistry, 1970
- Reaction of Fluorescein-Isothiocynate with Proteins and Amino AcidsThe Journal of Biochemistry, 1969
- Reaction of Fluorescein-Isothiocyanate with Proteins and Amino Acids: I. Covalent and Non-Covalent Binding of Fluorescein-Isothiocyanate and Fluorescein to ProteinsThe Journal of Biochemistry, 1969
- A Protein SequenatorEuropean Journal of Biochemistry, 1967
- Anion exchange chromatography of oxidized insulin peptidesJournal of Chromatography A, 1966