Effect of melanin on enzymatic hydrolysis of cellulosic waste

Publisher Website
Google Scholar
Abstract
Wood waste powder from Tectona grandis containing melanin was less susceptible to enzymatic hydrolysis than powder without melanin. About a 53% increase in saccharification was noted when melanin was removed. Melanin caused inhibition to all cellulolytic enzymes, but in different degrees. Endo‐β‐1,4‐glucanase and β‐glucosidase were markedly inhibited when melanin was preincubated with enzyme, while exo‐β‐1,4‐glucanase was severely inhibited when melanin was preincubated with substrate. The latter was found to be dependent on the contact time. The activities of endo‐β‐1,4‐glucanase and β‐glucosidase were noncompetitively inhibited by melanin.