Human B-cell lines constitutively express and secrete interleukin-16
Open Access
- 1 February 2000
- journal article
- research article
- Published by Wiley in Immunology
- Vol. 99 (2) , 266-271
- https://doi.org/10.1046/j.1365-2567.2000.00959.x
Abstract
Interleukin-16 (IL-16), produced by activated CD8+ T lymphocytes, is inhibitory to human immunodeficiency virus-1 (HIV-1) replication. In an attempt to determine whether human B cells express and secrete IL-16, a wide panel of B-cell lines derived from patients with acquired immune deficiency syndrome (AIDS)-associated B-cell lymphomas (AABCL) (n = 5) and from non-AABCLs (n = 8) were studied. Using reverse transcription–polymerase chain reaction (RT–PCR) analysis, we were able to observe ubiquitous expression of IL-16 mRNA. Kinetic studies on constitutive mRNA turnover and secretion for IL-16 suggests that the optimum expression is at 24 hr. Interestingly, we report, for the first time, IL-16 secretion by human B-cell lines.Keywords
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