Radioimmunoassay of Iodinated Tobramycin

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Abstract
A radioimmunoassay for tobramycin was developed using antisera from rabbits injected with a tobramycin-bovine serum albumin conjugate. The antibody had a relatively high affinity for 125 I-labeled tobramycin (1.6 × 10 9 liters/mol), thus indicating little interference in immunoreactivity by iodination. The radioimmunoassay was highly specific, and only minor cross-reaction occurred with other aminoglycoside antibiotics. The radioimmunoassay was also more sensitive than previously published tobramycin assays (minimum sensitivity, 280 pg), and there was a significant correlation ( P < 0.05) with both the radioenzymatic and microbiological assays.