Influence of Different Carbon Sources on Invertase Activity and Growth of Sour Cherry (Prunus cerasusL.) Shoot Cultures

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Abstract
The influence of different sugars on shoot multiplication in vitro and on the activity of invertase was studied with sour cherry (Prunus cerasus L.) cultures. The sugars sucrose, glucose and fructose, and the sugar alcohol sorbitol, were investigated at a wide range of concentrations. The optimum concentrations of all carbon sources were 2% and 3% (w/v). Sucrose and glucose favoured a similar rate of proliferation. However, in the presence of fructose, proliferation was lowest but was coupled with the highest frequency of formation of long shoots. The highest activity of total invertase was for tissues growing on a sucrose-containing medium while, in a sugar-free medium, invertase activity was mainly found in the ‘salt extracted’ fraction. In the remaining treatments, ‘soluble invertase’ dominated. For each sugar investigated, the activity of both forms of invertase was significantly higher at 2% than at 3% (w/v) sugar.

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