A Barley Endonuclease Specific for Apurinic DNA
Open Access
- 28 June 1978
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 87 (2) , 215-220
- https://doi.org/10.1111/j.1432-1033.1978.tb12368.x
Abstract
An endonuclease specific for depurinated native DNA was isolated and partially purified from extracts of barley leaves. The procedure included streptomycin sulphate precipitation. ammonium sulphate fractionation, phosphocellulose, hydroxyapatite and Sephadex G-150 chromatography. Purityo of the resulting enzyme was determined by gel electrophoresis and gel chromatogrphy and specificity be testing the activity on intact and depurinated bacterial DNAs. At lower concentrations. the enxyme is specific for DNA containing apurinic sites. At higher concentrations, however, it degrades DNA in a non-specific manner. the nuclease has a pH optimum at 7.6, and a molecular weight of about 18000.This publication has 28 references indexed in Scilit:
- Isolation from barley embryo of endonuclease specific for apurinic sites in DNABiologia plantarum, 1977
- DNA repair synthesis stimulated by mutagenic N-methyl-N-nitrosourea in barley seeds and free embryosMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1977
- Endonucleases for apurinic sites in plantsFEBS Letters, 1976
- Human endonuclease activity for DNA apurinic sitesNature, 1975
- Mammalian DNA endonuclease acting at apurinic sites: Absence of associated exonuclease activityFEBS Letters, 1975
- Purification and some properties of a mammalian repair endonucleaseBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- Nuclease for DNA Apurinic Sites May be Involved in the Maintenance of DNA in Normal CellsNature New Biology, 1973
- Repair of single-strand breaks and fate of N-7-methylguanine in DNA during the recovery from genetical damage induced by N-methyl-N-nitrosourea in barley seedsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1973
- Rate of depurination of native deoxyribonucleic acidBiochemistry, 1972
- Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gelsBiochemical and Biophysical Research Communications, 1967