Effect of fixation time on measurement of 2′-deoxyribonucleoside 5′-triphosphate pools in the rat embyro
- 1 June 1979
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 179 (3) , 715-717
- https://doi.org/10.1042/bj1790715
Abstract
Values for the concentrations of deoxyribonucleoside triphosphates in rat embryos on day 12 of gestation, determined by high-pressure liquid chromatography, were artifactually two to three times as high in embryos fixed by cooling in ice/water followed by freezing on solid CO2, in 20s, as in those more rapidly/fixed in liquid N2, in 1 s.This publication has 15 references indexed in Scilit:
- Regional levels of choline and acetylcholine in rat brain following head focussed microwave sacrifice: Effect of (+)-amphetamine and (±)-parachloroamphetamineNeuropharmacology, 1976
- Factors determining cell sensitivity to methotrexate: Studies of folate and deoxyribonucleoside triphosphate pools in five mammalian cell linesPublished by Elsevier ,1974
- Cell-cycle-dependent variations of deoxyribonucleoside triphosphate pools in chinese hamster cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- FREEZE‐BLOWING: A NEW TECHNIQUE FOR THE STUDY OF BRAIN IN VIVOJournal of Neurochemistry, 1973
- Cyclic Adenosine Monophosphate in Brain Areas: Microwave Irradiation as a Means of Tissue FixationScience, 1971
- Teratogenesis and inhibition of DNA synthesis induced in rat embryos by cytosine arabinosideTeratology, 1971
- The ATP pool in Escherichia coli. I. Measurement of the pool using a modified luciferase assayBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1967
- Nucleotide pools and [6-14C]orotic acid incorporation in early regenerating rat liverBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966
- Free Nucleotides in Animal TissuesProgress in Nucleic Acid Research and Molecular Biology, 1964
- FREE NUCLEOTIDES OF THE BRAIN IN VARIOUS MAMMALSJournal of Neurochemistry, 1961