A CHEMICAL PROCEDURE FOR DETERMINATION OF THE C14-DISTRIBUTION IN LABELLED XYLOSE

Publisher Website
Google Scholar
Abstract
A series of reactions reported previously for the degradation of glucose was modified and extended to permit the determination of C14 in each of the 5 carbons of a single 2 m[image] xylose sample. Methyl xylopyranoside was oxidized with periodic acid giving C-3 as formic acid, and a dialdehyde which was converted to strontium methoxy-diglycolate. The purified salt was hydrolyzed to glyoxylic and glycolic acids. The glyoxylic acid was isolated as the 2,4-dinitrophenylhydrazone (C-1 + C-2) which was decarboxy-lated to give CO2 from C-2. The glycolic acid was oxidized by lead tetraacetate to give C-4 as CO2 and C-5 as formaldehyde. The activity in C-l was determined by difference. The method was applied to xylose-1-C14, xylose-5-C14, and a biologically synthetized xylose sample with satisfactory results. This degradation procedure is theoretically applicable to other aldo-pentoses and aldotetroses.

This publication has 2 references indexed in Scilit: