The phage RNA polymerases are related to DNA polymerases and reverse transcriptases
- 27 October 1993
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 10 (1) , 1-6
- https://doi.org/10.1111/j.1365-2958.1993.tb00897.x
Abstract
The single subunit DNA-dependent RNA polymerase (RNAP) that is encoded by bacteriophage T7 is the prototype of a class of relatively simple RNAPs that includes the RNAPs of the related phages T3 and SP6, as well as the mitochondrial RNAPs. The T7 enzyme has been crystallized, and recent genetic and biochemical analyses have facilitated an interpretation of this structure. A growing body of evidence suggests that the phage-like RNAPs are related to other nucleotide polymerases such as DNA polymerases, RNA-dependent RNA polymerases, and reverse transcriptases. In this work, we review information concerning the structure and function of T7 RNAP, and evidence in support of its assignment to a broader class of nucleotide polymerases.Keywords
This publication has 44 references indexed in Scilit:
- Substitution of a single bacteriophage T3 residue in bacteriophage T7 RNA polymerase at position 748 results in a switch in promoter specificityJournal of Molecular Biology, 1992
- Asp537, Asp812 are essential and Lys631, His811 are catalytically significant in bacteriophage T7 RNA polymerase activityJournal of Molecular Biology, 1992
- Isolation and characterization of mutant bacteriophage T7 RNA polymerasesJournal of Molecular Biology, 1992
- Model for the mechanism of bacteriophage T7 RNAP transcription initiation and terminationJournal of Molecular Biology, 1992
- DNA-dependent RNA polymerase from bacteriophage T3 transcribes and amplifies an RNA template in vitroGene, 1991
- Processivity of T7 RNA polymerase requires the C-terminal Phe882-Ala883-COO- or "foot"Biochemistry, 1991
- An attempt to unify the structure of polymerasesProtein Engineering, Design and Selection, 1990
- T7 lysozyme inhibits transcription by T7 RNA polymeraseCell, 1987
- Transcription by T7 RNA polymerase is not zinc-dependent and is abolished on amidomethylation of cysteine-347Biochemistry, 1986
- Nucleotide sequence of the gene for bacteriophage T7 RNA polymeraseJournal of Molecular Biology, 1984