Single Ca channel currents in mouse pancreatic B-cells

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Abstract
Barium currents flowing through single Ca2+ channels were recorded from outside-out patches isolated from mouse pancreatic B-cells. Only one type of Ca2+ channel was observed. In 110 mM Ba2+, the single channel conductance was 24 pS (at negative membrane potentials) and the current amplitude at 0 mV was−0.7 pA. Channel openings were activated by depolarisations more positive than −30 mV and showed little inactivation during 200 ms pulses. Open times were increased by BAY K 8644 an decreased by micromolar Cd2+. Channel activity was subject to rundown in excised patches and little activity remained after 10 min. These properties resemble those of L-type Ca2+ channels in other tissues. It is suggested that this Ca2+ channel participates in the generation of the B-cell action potential and mediates the increase in Ca2+ influx required for insulin secretion.