Purification and properties of ascorbate free-radical reductase from potato tubers
- 1 April 1986
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 167 (4) , 521-526
- https://doi.org/10.1007/bf00391228
Abstract
Ascrobate free-radical reductase (EC 1.6.5.4) from potato tubers was purified to apparent homogencity by a method which included ammonium-sulfate precipitation, gel filtration and chromatography on diethylaminoethyl cellulose and hydroxylapatite. Gel filtration and gel electrophoresis showed that the purified enzyme was monomeric with a molecular weight of about 42 000. Enzyme activity was heat lable and severely inhibited by thiol reagents. The Km values for enzyme substrates were estimated.Keywords
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