Simultaneous analysis of prostanoids using liquid chromatography/high‐field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry
- 20 April 2006
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 20 (10) , 1504-1510
- https://doi.org/10.1002/rcm.2505
Abstract
A liquid chromatography/high-field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry (LC-FAIMS-MS/MS) semi-quantitative method was developed for the simultaneous determination of prostaglandin (PG) E2, PGD2, PGF2α, 6-keto-PGF1α, and thromboxane (TX) B2. Diluted samples containing these prostanoids and their tetra-deuterium-substituted internal standards were analyzed by LC followed by either selected reaction monitoring (LC-SRM) or FAIMS and selected reaction monitoring (LC-FRM). FAIMS reduced background noise, separated the isobaric ions PGE2 and PGD2, and separated dynamically interchanging TXB2 anomers. This is the first report of the separation of interconverting anomers by FAIMS. Generally, the LC-FRM chromatograms were more selective than the LC-SRM chromatograms. Its potential was demonstrated by analysis of prostanoids in guinea pig lumbar spinal cord homogenate. Copyright © 2006 John Wiley & Sons, Ltd.Keywords
This publication has 26 references indexed in Scilit:
- LC–MS/MS-analysis of prostaglandin E2 and D2 in microdialysis samples of ratsJournal of Chromatography B, 2005
- Removal of metabolite interference during liquid chromatography/tandem mass spectrometry using high-field asymmetric waveform ion mobility spectrometryRapid Communications in Mass Spectrometry, 2005
- High-field asymmetric waveform ion mobility spectrometry: A new tool for mass spectrometryJournal of Chromatography A, 2004
- Separation of β2‐microglobulin conformers by high‐field asymmetric waveform ion mobility spectrometry (FAIMS) coupled to electrospray ionisation mass spectrometryRapid Communications in Mass Spectrometry, 2004
- Carrageenan-induced Paw Edema in Rat Elicits a Predominant Prostaglandin E2 (PGE2) Response in the Central Nervous System Associated with the Induction of Microsomal PGE2 Synthase-1Journal of Biological Chemistry, 2004
- Deletion of Microsomal Prostaglandin E2 (PGE2) Synthase-1 Reduces Inducible and Basal PGE2 Production and Alters the Gastric Prostanoid ProfileJournal of Biological Chemistry, 2004
- Liquid chromatographic–mass spectrometric determination of cyclooxygenase metabolites of arachidonic acid in cultured cellsJournal of Chromatography B, 2003
- Reversed-phase high-performance liquid chromatography for simultaneous determination of prostaglandins E2, A2 and B2International Journal of Pharmaceutics, 1994
- Formation of Isoindole Derivatives of Sulfidopeptide Leukotrienes by Reaction with O-Phthalaldehyde and Separation by Reverse Phase High Performance Liquid ChromatographyJournal of Liquid Chromatography, 1987