Sodium fluoride is a less efficient human cell mutagen at low concentrations
- 1 January 1990
- journal article
- research article
- Published by Wiley in Environmental and Molecular Mutagenesis
- Vol. 15 (2) , 71-77
- https://doi.org/10.1002/em.2850150203
Abstract
Sodium fluoride was found to induce gene‐locus mutations at the thymidine kinase (tk) and hypoxanthine guanine phosphoribosyl transferase (hgprt) loci in human lymphoblastoid cells. A single, 28 hr exposure to up to 600 μg/ml sodium fluoride induced a concentrationdependent increase in mutant fraction at both gene loci and reduced cell survival to 12% relative to negative control cultures. When cells were exposed to sodium fluoride concentrations that were only minimally toxic using a 20 day treatment protocol, no detectable induction of mutation was ob‐served at the the hgprt locus, and induction of mutation was observed at the tk locus only for treatment with 65 μg/ml sodium fluoride; exposure to 50 and 35 μg/ml sodium fluoride did not induce detectable mutation. The assay protocol used was of sufficient statistical sensitivity to detect the level of mutation predicted based on a linear extrapolation of data obtained from a 28 hour exposure. The implications of these observations with regard to the extrapolability of mutagenicity data to low concentrations are discussed.Keywords
This publication has 21 references indexed in Scilit:
- Long-term, low-dose benzo[α]pyrene-induced mutation in human lymphoblasts competent in xenobiotic metabolismMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1989
- Analysis of human lymphoblast mutation assays by using historical negative control data basesEnvironmental and Molecular Mutagenesis, 1986
- Alterations of the hprt gene in human in vivo-derived 6-thioguanine-resistant T lymphocytesNature, 1985
- In vivo somatic mutations in human lymphocytes frequently result from major gene alterationsNature, 1985
- Assay for gene mutation in a human lymphoblast line, AHH-1, competent for xenobiotic metabolismMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1984
- Cytotoxicity, chromosome aberrations and unscheduled DNA synthesis in cultured human diploid fibroblasts induced by sodium fluorideMutation Research Letters, 1984
- Mutation of human lymphoblasts exposed to low concentrations of chemical mutagens for long periods of timeMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1983
- The uracil–fluoride interaction: ab initio calculations including solvationJournal of the Chemical Society, Chemical Communications, 1982
- Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: Possibility for a rapid human cell mutation assayBiochemical and Biophysical Research Communications, 1978
- A review of systemic and topical fluorides for the prevention of dental cariesCommunity Dentistry and Oral Epidemiology, 1973