Interaction of HIV‐1 with susceptible lymphoblastoid cells 1H NMR studies
- 8 July 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 285 (1) , 11-16
- https://doi.org/10.1016/0014-5793(91)80713-d
Abstract
Different strains of HIV susceptible lymphoblastoid cells have been infected by HIV-1 and examined by means of 1H NMR spectroscopy at different times after infection, taking advantage of the presence of high resolution lipid signals from the plasma membrane of tumor cells. A transient decrease in intensity of fatty acid signals, originated by changes in membrane structure, has been observed early after viral infection. Marked alterations in membrane-dependent steps of phospholipid synthesis can also be inferred by the observed transient depression in peaks from choline-based metabolites. Spectral modifications deriving from charges in lipid metabolism are also produced both in infected cells a few days after infection and in permanently infected cells. 1H NMR can, therefore, monitor structural and metabolic effects induced by HIV infectionKeywords
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