Penetration of cefotaxime and desacetylcefotaxime into skin blister fluid

Abstract
Serum and suction skin blister fluid concentrations of cefotaxime (CT), and its metabolite, desacetylcefotaxime (DACT), were measured in four healthy volunteers during the first 10 h after an intravenous dose of 1·0 g CT. The blisters were produced by suction applied to the forearm skin prior to drug administration. The antibiotic concentrations were assayed by high-pressure liquid chromatography. The ratio of the areas under the concentration vs. time curves for blister fluid and serum (AUCblister/AUCserum) of DACT was nearly twice that of CT. This suggests a better penetration into skin blister fluid of the metabolite. The elimination half-life of CT was shorter in serum (0·85 h) than in blister fluid (3·0 h), whereas the difference was less for DACT (1·5 h vs. 3·8 h).

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