Abstract
The correlation between accumulation of linolenic acid in the crowns and roots of winter wheat and its frost hardening at low temperature is indirect. It results from their common requirement for light and low temperature. Light mainly produces energy and carbon reserves. The partial hardening obtained in darkness could be eliminated by preetiolation. The high levels of linolenic acid and frost resistance reached after hardening were maintained for a long time in darkness. The levels of linolenic acid and frost hardiness decreased faster at low temperature in hardened plants after treatment with BASF 13-338, a substituted pyridazinone, than in plants deprived of light. The mode of action of BASF 13-338 is apparently not limited to the inhibition of photosynthesis.