Direct measurement of antigens in serum by time-resolved fluoroimmunoassay.
Open Access
- 1 January 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 31 (1) , 50-53
- https://doi.org/10.1093/clinchem/31.1.50
Abstract
A long-lived fluorescence label (Tb3+) has been attached to the antigen of interest by using a bifunctional chelating agent 1-(p-benzenediazonium)-EDTA. A nonequilibrium competitive-binding immunoassay protocol, in conjunction with time-resolved detection of the long-lived fluorescence label, allows the antigen to be analyzed directly in samples containing diluted human serum. Results obtained for immunoglobulin G with this simple and rapid procedure correlated well (r = 0.93) with those by a commercially available fluorescence immunoassay method.This publication has 3 references indexed in Scilit:
- Time-resolved fluoroimmunoassay of human pancreatic phospholipase A2.Clinical Chemistry, 1983
- Time-resolved fluorometer for lanthanide chelates--a new generation of nonisotopic immunoassays.Clinical Chemistry, 1983
- Time-resolved fluoroimmunoassay of human choriogonadotropin.Clinical Chemistry, 1983