The Determination of Optical Isomers of 2-[3-(2-Chloro-Penoxyphenyl)]-Propionic Acid in Rat Plasma by High Performance Liquid Chromatography

Abstract
The optical isomers of dl-2-[3-(2-chlorophenoxy-phenyl)]-propionic acid in rat plasma is converted to the diastereomeric derivatives with (+)-2-aminobutane and then determined by high performance liquid chromatography using a Nucleosil NH2 (10 μm) column and cyclohexane-ethyl acetate (5:1) as a mobile phase. The time course of the optical isomers of this drug in rat plasma after oral administration was measured. The proposed method is specific and reproducible for the determination of the optical isomers of this drug.