Preparation and Optimization of a Cell-free Translation System fromVicia sativaGerm Lacking Ribosome-inactivating Protein Activity
- 1 May 1992
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 43 (5) , 729-737
- https://doi.org/10.1093/jxb/43.5.729
Abstract
A cell-free translation system from Vicia sativa with high activity for reading endogenous messengers was prepared. The concentrations of several of the components were optimized. The system was highly sensitive to cycloheximide, puromycin and fusidic acid, but was completely insensitive to chloramphenicol. The system lacks apparent free endogenous ribosome-inactivating protein activity. It was highly sensitive to several well-known ribosome-inactivating proteins, which makes it very suitable for studying the effects and the mechanism of action of these plant toxins.Keywords
This publication has 0 references indexed in Scilit: