A “LOOP” METHOD FOR COUNTING VIABLE BACTERIA OR BACTERIOPHAGE
- 1 February 1951
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Medical Sciences
- Vol. 29 (1) , 1-4
- https://doi.org/10.1139/cjms51-001
Abstract
A “loop” method for counting viable bacteria or bacteriophage is described. In this method standard wire loops are used to transfer definite volumes of cultures in two dilution tubes and to spread fluid from the final dilution on an agar plate. Instead of a number of tubes and pipettes that must be laboriously cleaned, plugged, and sterilized, the only requirements are two sterile tubes containing 20 ml. of saline and two wire loops. The loops are sterilized by flaming.Keywords
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