Auxin Biogenesis

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Abstract
Subcellular fractionation of cucumber (Cucumis sativus L.) seedlings was achieved, and 2 of the enzymes in the auxin biosynthetic pathway were localized. NADH-specific indoleacetaldehyde reductase activity was observed only in the cytosol fractions obtained from separated hypocotyl and cotyledon tissue. In contrast, a portion of the NADPH-specific indoleacetaldehyde reductase activity was associated with a microsomal fraction derived from these tissues. The NADPH-specific indoleacetaldehyde reductase was more firmly associated with the microsomal fraction derived from hypocotyls than with that from the cotyledons. These resutls indicate a division of the terminal steps of auxin biogenesis into at least 2 subcellular compartments.