A facile method for the preparation of N6-substituted ATP-Sepharose
- 1 January 1975
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (10) , 1771-1776
- https://doi.org/10.1093/nar/2.10.1771
Abstract
The attachment of 6-methylsulfonylpurineribose 5'-triphosphate to AH-Sepharose 4B as a facile method for the preparation of ATP-Sepharose is described. This reaction is compared to the coupling of 6-chloropurineriboside 5'-triphosphate as well as N(6)-aminocaproyl-ATP. An improved synthesis of purineriboside 5'-phosphates which does not require purification by column chromatography is also described.Keywords
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