Radioimmunoassay of somatomedin-C in filter paper discs containing dried blood.

31 March 1987

journal article
research article
Published by Oxford University Press (OUP) in Clinical Chemistry

Vol. 33 (4) , 536-538
https://doi.org/10.1093/clinchem/33.4.536

Abstract

We describe a simple radioimmunoassay (RIA) for estimating concentrations of somatomedin-C (Sm-C) in dried blood on filter paper. A single 3.2-mm blood spot specimen on filter paper is eluted overnight into buffer containing antibody and 125I-labeled Sm-C. The following day, bound and free hormones are separated by addition of goat anti-rabbit gamma globulin in 60 g/L polyethylene glycol solution. The correlation between values obtained for such blood-spot discs and the corresponding wet plasma is highly significant (r = 0.90, p less than 0.001). The relative concentrations (arbitrary units) of Sm-C as determined for specimens on filter paper from mothers and infants, and for cord bloods, are similar to those reported by others using acidified serum.

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