Modification of the Reichert FC‐2 cryoattachment to improve ultrathin sectioning reproducibility
- 1 December 1979
- journal article
- Published by Wiley in Journal of Microscopy
- Vol. 117 (3) , 355-362
- https://doi.org/10.1111/j.1365-2818.1979.tb04692.x
Abstract
Modifications of the Reichert FC-2 cryomicrotome attachment which facilitate sectioning of frozen material are described. Most valuable was the construction of a specimen holder chuck made of brass and connected to the specimen arm with a glass rod. Other changes include improved shielding from heat influx, a constant nitrogen pressure cooling system and a stable cryochamber mount. These modifications considerably improve reproducibility.Keywords
This publication has 3 references indexed in Scilit:
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- A TECHNIQUE FOR ULTRACRYOTOMY OF CELL SUSPENSIONS AND TISSUESThe Journal of cell biology, 1973
- IMPROVED TECHNIQUES FOR THE PREPARATION OF ULTRATHIN FROZEN SECTIONSThe Journal of cell biology, 1971