Methylene Selectivity and Eluotropic Strength Variations in Subcritical Fluid Chromatography with Packed Columns and CO2-Modifier Mobile Phases
- 4 May 1999
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 71 (11) , 2164-2170
- https://doi.org/10.1021/ac9813944
Abstract
This work has consisted of better understanding the chro-matographic behavior in subcritical fluid chromatography with CO2−acetonitrile and CO2−methanol mobile phases. To that end, the changes of solute solubility in the mobile phase have been studied by determination of methylene selectivity of a homologous series. Moreover, eluotropic strengths have been calculated for mobile phases containing from 5 to 45% modifier. To carry out the studies in all ranges of the subcritical area, experimental designs have been used. The influences of analytical temperature (from 10 to 30 °C) and outlet pressure (from 10 to 20 MPa) have also been investigated both on solubility and on eluotropic strength. A more general comparison of elu-otropic strengths of subFC and HPLC has been achieved.Keywords
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