Thermostability of bacterial luciferase expressed in different microbes
- 1 August 1994
- journal article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 77 (2) , 149-154
- https://doi.org/10.1111/j.1365-2672.1994.tb03058.x
Abstract
Bacterial luciferase was used to investigate the relationship between the thermostability of a cytoplasmic reporter molecule and cellular heat resistance. The luciferase activity of Vibrio fischeri was expressed in strains of Escherichia coli, Salmonella typhimurium, Listeria monocytogenes and Brochothrix thermosphacta following transformation with plasmid pSP13 carrying the luxAB genes. The thermostability of intracellular luciferase varied depending on the organism in which it was expressed, but was not related to the cellular heat resistance of the different organisms. Addition of xylitol to the heating medium protected against loss of viability and inactivation of intracellular luciferase. Glycerol also protected against loss of viability but was less effective at preventing thermal denaturation of luciferase.Keywords
This publication has 14 references indexed in Scilit:
- Heterologous gene expression inCampylobacter coli: The use of bacterial luciferase in a promoter probe vectorFEMS Microbiology Letters, 1993
- Thermal denaturation and loss of viability in Escherichia coli and Bacillus stearothermophilusLetters in Applied Microbiology, 1993
- Reduced ribosomal thermal denaturation inListeria monocytogenesfollowing osmotic and heat shocksFEMS Microbiology Letters, 1993
- The use of bacterial luciferase for monitoring the environmental regulation of expression of genes encoding virulence factors in Listeria monocytogenesJournal of General Microbiology, 1992
- Thermal denaturation of whole cells and cell components of Escherichia coli examined by differential scanning calorimetryJournal of General Microbiology, 1991
- Thermal inactivation of Listeria monocytogenes studied by differential scanning calorimetryJournal of General Microbiology, 1991
- A submerged-coil heating apparatus for investigating thermal inactivation of micro-organismsLetters in Applied Microbiology, 1990
- The Effect of Sugars and Polyols on the Heat Resistance of SalmonellaeJournal of Applied Bacteriology, 1974
- A Note on the Development of Resistance to Heat and Gamma Radiation in SalmonellaJournal of Applied Bacteriology, 1970
- The Sodium and Potassium Content of Non-Halophilic Bacteria in Relation to Salt ToleranceJournal of General Microbiology, 1961