Growth Characteristics of Acute Hemorrhagic Conjunctivitis (AHC) Virus in Monkey Kidney Cells

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Abstract
Effect of temperature on growth in monkey kidney (MK) cells of acute hemorrhagic conjunctivitis (AHC) virus was studied. The optimal growth temperature ranged from 32 to 34°, and 39° was nonpermissive. In one-step growth experiments at 33°, the standard strain J 670/71 of AHC virus grew exponentially after a latent period of 2 h, and reached a maximum titer within 6 h post-infection (p.i.). The temperature-shift experiment showed that incubation at 39° during the latent period of the growth cycle had little effect on the subsequent viral growth at 33°, and when the shift-down was carried out at 2 h p. i. or later, viral growth immediately started and reached a maximum. Conversely, viral growth at 33° was immediately stopped by shift-up to the nonpermissive temperature. The shift-down experiment using guanidine-HCl suggested a lack of progeny RNA synthesis at the nonpermissive temperature.

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