An improved procedure for the X-ray microanalysis of acid phosphatase activity in lysosomes
- 1 January 1987
- journal article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 87 (1) , 71-77
- https://doi.org/10.1007/bf00518727
Abstract
The useful detection of acid phosphatase activity with cerium as a capturing agent is confirmed. By introducing a freeze step in combination with a preincubation, reliably localized, lysosomal precipitates are obtained and aspecific ones prevented. Short (t4 plus K4Fe (CN)6 or OsO4 plus aminotriazole, added to lysosomal cerium localization a high membrane contrast. The detection of cerium by X-ray microanalysis is improved by a better spectral separation of the osmium (M α) and cerium (L α) peaks.Keywords
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